High-level expression of Bacillus naganoensis pullulanase from recombinant Escherichia coli with auto-induction: effect of lac operator.
Pullulanase plays an important role in specific hydrolysis of branch points SAMe in amylopectin and is generally employed as an important enzyme in starch-processing industry.So far, however, the production level of pullulanase is still somewhat low from wide-type strains and even heterologous expression systems.Here the gene encoding Bacillus naga